Application

Research Sub CategoryApoptosis - Additional

Research CategoryInflammation & Immunology

Detect mA20/TNFAIP3 using this Anti-mA20 Antibody/TNFAIP3, clone 37 validated for use in flow cytometry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

TNFAIP3/mA20, also known as Tumor necrosis factor alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, or Zinc finger protein A20, and encoded by the gene TNFAIP3/OTUD7C, is an ubiquitin editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. TNFAIP3/mA20 is involved in immune and inflammatory responses signaled by cytokines or pathogens through the Toll-like receptors and ultimately regulating NF-kappa-B activity. TNFAIP3/mA20 is an essential component of the ubiquitin editing protein complex consisting of RNF11, ICTH, and TAX1BP1 and ensures the transient nature of inflammatory signaling. TNFAIP3/mA20 is also a key player in switching of Lys-63 polyubiquitinated chains to Lys-48 polyubiquitinated chains for the subsequent degradation of specific targets as well. TNFAIP3/mA20 is located in the cytoplasm but can be found in the nucleus and lysosome depending upon the microenvironment and stimulus.

Immunogen

Recombinant protein corresponding to Mouse mA20/TNFAIP3.

Physical form

Purified rat monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Purified by Ion-Exchange Chromatography

Format: Purified

Quality

Evaluated by Flow Cytometry in mouse splenocytes.Flow Cytometry Analysis: 2 µg of this antibody detected mA20/TNFAIP3 in 1X10E6 mouse splenocytes.This antibody was produced in collaboration with the Scripps Research Institute.For Scripps Research Institute Validation (Click Here)

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

90 kDa calculated